A Simple Key For thr77 Unveiled
A Simple Key For thr77 Unveiled
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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has become noticed in many tumors, which leads to chromosome instability, and thus might contribute to tumorigenesis. This protein was observed to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a significant role in endorsing mobile-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are already described. Two additional splice variants have been described but comprehensive nucleotide sequence details is not really nonetheless readily available.
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Blocking peptides are peptides that bind particularly to your focus on antibody and block antibody binding. These peptide ordinarily consists of the epitope regarded because of the antibody.
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This information outlines additional doable optimization of mobile and immunoassay parameters to guarantee the very best results are...
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This manual outlines even more attainable optimization of mobile and immunoassay parameters to make certain the very best benefits are...
Antibodies sure to the blocking peptide now not bind to the epitope within the focus on protein. By comparing the staining through the blocked antibody compared to the antibody on your own, you can see which staining is precise.
Concentrate on Information and facts Cyclin E belongs for the highly conserved cyclin family members, whose customers are characterized by a remarkable periodicity in protein abundance throughout the mobile cycle. Cyclins purpose as regulators of CDK kinases. Different cyclins show distinctive expression and degradation designs which add into the temporal coordination of every mitotic party. Cyclin E kinds a posh with and features as being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S section boundary and is degraded as cells progress by S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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